A1 Journal article – refereed
Construction of antibody mimics from a noncatalytic enzyme-detection of polysialic acid




List of Authors: Jokilammi A, Ollikka P, Korja M, Jakobsson E, Loimaranta V, Haataja S, Hirvonen H, Finne J
Publication year: 2004
Journal: Journal of Immunological Methods
Volume number: 295
Issue number: 1-2

Abstract

We have used a conceptually novel way to construct antibody mimics based
on the binding of a noncatalytic enzyme to its substrate.
Bacteriophage-derived endosialidase cleaves polysialic acid (polySia),
an important oncofetal and bacterial antigen, which is poorly
immunogenic. We fused to green fluorescent protein (GFP) a catalytically
inactive endosialidase known to bind but not degrade polysialic acid.
The fusion protein is a convenient single-step reagent in fluorescence
microscopy, binding assays and immunoblots. It efficiently and
specifically detected polysialic acid in developing brain, neuroblastoma
cells and bacteria causing meningitis. Enzyme-substrate interactions
represent an unexploited source of molecular recognition events. Some of
these could be used in designing well-defined substitute antibodies for
the study of target molecules which are difficult to purify, available
in low quantities, are unstable or have poor immunogenity.


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