A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

Ouabain is not detectable in human plasma




TekijätLewis LK, Yandle TG, Lewis JG, Richards AM, Pidgeon GB, Kaaja RJ, Nicholls MG

Julkaisuvuosi1994

JournalHypertension

Tietokannassa oleva lehden nimiHypertension (Dallas, Tex. : 1979)

Lehden akronyymiHypertension

Vuosikerta24

Numero5

Aloitussivu549

Lopetussivu55

Sivujen määrä7

ISSN0194-911X

DOIhttps://doi.org/10.1161/01.HYP.24.5.549


Tiivistelmä
An enzyme-linked immunosorbent assay is described for the measurement of ouabain in human plasma. This assay is specific for ouabain, strophanthidin, and ouabagenin, with other steroids, including digoxin and vasopressor hormones, exhibiting negligible cross-reactivity. Assay sensitivity was 0.06 nmol/L if 1 mL plasma was extracted and less than 0.005 nmol/L when 20 mL plasma was analyzed. Extracted plasma samples showed ouabainlike immunoreactivity that diluted in parallel with the ouabain standard curve. Repeated extraction and assay of single plasma samples, however, did not produce consistent results in the assay. Increased specificity was obtained by high-performance liquid chromatography of sample extracts before assay. When high-performance liquid chromatographic profiles of plasma spiked with ouabain standard or following bolus intravenous injections of ouabain into normal human volunteers were compared with profiles of unspiked plasma, there was no support for the immunoreactive material in the latter samples being ouabain. We propose that if ouabain is present in the human circulation, its concentration is less than 0.005 nmol/L.



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