A1 Journal article – refereed
Regulation of gingival epithelial cytokine response by bacterial cyclic dinucleotides




List of Authors: Samira Elmanfi, Jie Zhou, Herman O. Sintim, Eija Könönen, Mervi Gürsoy, Ulvi Kahraman Gürsoy
Publisher: Taylor&Francis
Publication year: 2019
Journal: Journal of Oral Microbiology
Volume number: 11
Issue number: 1

Abstract

Background: Cyclic dinucleotides (cyclic di-guanosine monophosphate
(c-di-GMP) and cyclic di-adenosine monophosphate (c-di-AMP)) and
lipopolysaccharides (LPS) are pathogen-associated molecular patterns
(PAMPs). Individual impacts of PAMPs on immune system have been
evaluated, but simultaneous actions of multiple PAMPs have not been
studied.

Objective: Examination the effects of cyclic dinucleotides and Porphyromonas gingivalis
LPS on gingival epithelial cytokine response.

Methods: Human gingival
keratinocytes (HMK) were incubated with 1, 10, and 100 µM concentrations
of c-di-GMP and c-di-AMP, either in the presence or absence of P. gingivalis
LPS. Intra- and extracellular levels of interleukin (IL)-1β, IL-8,
IL-1Ra, monocyte chemoattractant protein (MCP)-1, and vascular
endothelial growth factor (VEGF), were measured using the Luminex
technique.

Results: LPS decreased extracellular IL-8 levels, while the
presence of c-di-AMP inhibited this effect. Incubating HMK cells with
c-di-AMP (alone or with LPS) elevated the extracellular level of MCP-1.
Extracellular VEGF level increased when cells were incubated with LPS
and c-di-GMP together, or with c-di-AMP alone. LPS and c-di-AMP
suppressed intracellular IL-1β levels. The c-di-AMP elevated
intracellular levels of IL-1Ra.

Conclusion: c-di-AMP and, to a lesser
extent, c-di-GMP regulate keratinocyte cytokine response, either as an
aggregator or as a suppressor of LPS, depending on the cytokine type.


Downloadable publication

This is an electronic reprint of the original article.
This reprint may differ from the original in pagination and typographic detail. Please cite the original version.




Last updated on 2019-13-02 at 09:54