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Lineage-specific variations in the trigger loop modulate RNA proofreading by bacterial RNA polymerases




TekijätEsyunina D, Turtola M, Pupov D, Bass I, Klimašauskas S, Belogurov G, Kulbachinskiy A

KustantajaOxford Univ Press

Julkaisuvuosi2016

JournalNucleic Acids Research

Vuosikerta44

Numero3

Aloitussivu1298

Lopetussivu1308

Sivujen määrä11

ISSN0305-1048

DOIhttps://doi.org/10.1093/nar/gkv1521

Verkko-osoitehttp://nar.oxfordjournals.org/content/44/3/1298


Tiivistelmä

RNA cleavage by bacterial RNA polymerase (RNAP) has been implicated in transcriptional proofreading and reactivation of arrested transcription elongation complexes but its molecular mechanism is less understood than the mechanism of nucleotide addition, despite both reactions taking place in the same active site. RNAP from the radioresistant bacterium Deinococcus radiodurans is characterized by highly efficient intrinsic RNA cleavage in comparison with Escherichia coli RNAP. We find that the enhanced RNA cleavage activity largely derives from amino acid substitutions in the trigger loop (TL), a mobile element of the active site involved in various RNAP activities. The differences in RNA cleavage between these RNAPs disappear when the TL is deleted, or in the presence of GreA cleavage factors, which replace the TL in the active site. We propose that the TL substitutions modulate the RNA cleavage activity by altering the TL folding and its contacts with substrate RNA and that the resulting differences in transcriptional proofreading may play a role in bacterial stress adaptation.


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Last updated on 2024-26-11 at 12:35