A1 Journal article – refereed
3-Tesla Magnetic Resonance Angiographic Assessment of a Tissue-Engineered Small-Caliber Vascular Graft Implanted in a Rat

List of Authors: Yamanami M, Yamamoto A, Iida H, Watanabe T, Kanda K, Yaku H, Nakayama Y
Publisher: WILEY-LISS
Publication year: 2010
Journal: Journal of Biomedical Materials Research Part B: Applied Biomaterials
Journal acronym: J BIOMED MATER RES B
Volume number: 92B
Issue number: 1
Number of pages: 5
ISSN: 1552-4973

In the development of small-caliber vascular grafts (diameter; less than 3 mm), animal implantation studies have been mostly performed by using rat abdominal aortas, and their certain patency must evaluate with sacrificing every observation periods, which is both labor-intensive and time-consuming when performing a large number of experiments. This study is the first to demonstrate the application of 3-Tesla contrast-free time-of-flight magnetic resonance angiography (TOF-MRA) in the continuous assessment of the status of a tissue-engineered vascular graft in rat. As a model graft, a single connective tubular tissue (diameter; 1.5 mm), prepared by embedding the silicone rod (diameter; 1.5 mm) into a subcutaneous pouch of a rat for 2 weeks an in vivo tissue-engineering, was used. The graft was implanted in the abdominal aorta (diameter; 1.3 mm) of the rat by end-to-end anastomosis. Repeated TOF-MRA imaging of the graft obtained over a 3-month follow-up period after implantation made it possible to evaluate the patency of the graft, both simply and noninvasively. It also permitted visualization of the connected abdominal aorta and renal and common iliac arteries having smaller caliber (diameter; less than I mm). In addition, the degree of the stenosis or aneurysm could also be detected. 3-Tesla MRA allowed the simplified and noninvasive assessment of the status on the vascular graft, including the formation of a stenosis or aneurysm, in the same rat at different times, which will be contributing to enhance the development of tissue-engineered vascular grafts even with small caliber. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 92B: 156-160, 2010

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