A1 Journal article – refereed
Lipopolysaccharide-induced non-specific resistance to systemic Escherichia coli infection in mice

List of Authors: Vuopio-Varkila J, Nurminen M, Pyhälä L, Mäkelä PH
Publication year: 1988
Journal: Journal of Medical Microbiology
Journal name in source: Journal of medical microbiology
Journal acronym: J Med Microbiol
Volume number: 25
Issue number: 3
Number of pages: 7
ISSN: 0022-2615

A high degree of non-specific resistance to a lethal systemic Escherichia coli infection was induced in mice by pretreatment with a small dose (less than 5 micrograms/mouse) of the homologous lipopolysaccharide (LPS) or with heterologous rough-type LPS from E. coli K-12. The route of LPS administration, intraperitoneally or subcutaneously, did not influence the development of resistance, suggesting that a systemic cell activation was responsible for the effect. The enhanced elimination of bacteria was similar to that in mice recovering from a sublethal E. coli infection. In the LPS-treated mice, elimination of the challenge bacteria from the peritoneal cavity and the blood started 3-4 h after challenge whereas, in controls, the bacterial numbers continued to increase until the mice died. The detoxified LPS derivative, monophosphoryl lipid A (MPL), also increased the survival of mice infected with E. coli O18:K1. However, the dose of MPL required for optimal infection resistance was 100-fold greater than that of native, E. coli K-12 LPS, corresponding to the 100-fold reduced toxicity of MPL for mice and rabbits in lethality and pyrogenicity assays.

Last updated on 2019-21-08 at 21:42