Refereed journal article or data article (A1)

Diet-dependent gene expression highlights the importance of Cytochrome P450 in detoxification of algal secondary metabolites in a marine isopod




List of AuthorsPierre De Wit, Keith Yamada, Marina Panova, Carl André, Kerstin Johannesson

PublisherNature Publishing Group

Publication year2018

JournalScientific Reports

Journal acronymSci Rep

Article number16824

Volume number8

Number of pages8

ISSN2045-2322

eISSN2045-2322

DOIhttp://dx.doi.org/10.1038/s41598-018-34937-z

URLhttps://doi.org/10.1038/s41598-018-34937-z

Self-archived copy’s web addresshttps://research.utu.fi/converis/portal/detail/Publication/36411961


Abstract

Isopods of the genus Idotea have an unusual ability to feed on algae containing high amounts of chemical defense molecules, such as species of the genera Fucus and Ulva. In this study, we compared gene expression patterns of Idotea balthica individuals fed with Fucus vesiculosus to individuals fed with Ulva lactuca. We generated the first-ever transcriptome assembly for this species, and found 3,233 differentially expressed genes across feeding regimes. However, only a handful of biological functions were enriched with regard to differentially expressed genes, the most notable being “alkaloid metabolic process”. Within this category, we found eight differentially expressed cytochrome P450 (CYP) unigenes, all of which had a higher expression in the U. lactuca diet treatment. A phylogenetic analysis showed that the differentially expressed CYP genes are closely related to a CYP gene described from the hepatopancreas of the spiny lobster Panulirus argus, and we hypothesize that these transcripts are involved in metabolite detoxification. This is a first step in the understanding of this algae-grazer interaction, and will form a basis for future work to characterize cytochrome P450 functioning in marine crustaceans.


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Last updated on 2022-07-04 at 17:03