A1 Journal article – refereed
EFFECT OF NORADRENALINE ON THE METHEMOGLOBIN CONCENTRATION OF RAINBOW-TROUT RED-CELLS




List of Authors: PAAJASTE M, NIKINMAA M
Publisher: WILEY-LISS
Publication year: 1991
Journal: Journal of Experimental Zoology
Journal name in source: JOURNAL OF EXPERIMENTAL ZOOLOGY
Journal acronym: J EXP ZOOL
Volume number: 260
Issue number: 1
Number of pages: 5
ISSN: 0022-104X

Abstract


We studied the effect of noradrenaline on the methaemoglobin (metHb) concentration in rainbow trout red cells. The erythrocytes were incubated in physiological medium with or without noradrenaline and the percentage of metHb of total Hb content was measured. Noradrenaline lowered the metHb content significantly as compared to controls. To study if the effect of noradrenaline was caused by adrenergic intracellular alkalinization, cells were treated with noradrenaline + carbonic anhydrase or noradrenaline + acetazolamide. Carbonic anhydrase inhibits the adrenergic increase in intracellular pH, but did not reduce the effect of noradrenaline on the metHb concentration. Acetazolamide accentuates the increase in intracellular pH. However, there was no difference in the methaemoglobin content of noradrenaline-incubated and noradrenaline + acetazolamide-incubated cells. These results show that the effect of noradrenaline on the methaemoglobin content is independent from the adrenergic increase in intracellular pH. However, amiloride treatment inhibited the effect of noradrenaline on the methaemoglobin content, suggesting that the protein mediating sodium/proton exchange may also be involved in controlling cellular methaemoglobin levels.




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