Refereed journal article or data article (A1)

Polyomaviruses detectable in head and neck carcinomas




List of AuthorsSyrjänen S., Poluschkin L., Rautava J., Turunen A., Wang Y., Hedman K., Syrjänen K., Grenman R.

PublisherImpact Journals LLC

Publication year2018

JournalOncotarget

Journal name in sourceOncotarget

Volume number9

Issue number32

Start page22642

End page22652

ISSN1949-2553

DOIhttp://dx.doi.org/10.18632/oncotarget.25202

Self-archived copy’s web addresshttps://research.utu.fi/converis/portal/detail/Publication/30819813


Abstract

Polyomaviruses (PyV) independent or jointly with human papillomavirus (HPV), might have a role in head and neck carcinomas (HNSCC). We analyzed the prevalence and viral DNA loads of SV40, JCV and BKV with quantitative PCR (qPCR) and all 13 HPyVs with a novel Multiplex method in 82 HNSCC samples with known HPV status and disease-specific survival (DSS) and 24 HNSCC cell lines.

JCV was the most prevalent PyV present in 37% of HNSCC and the most prevalent sites were lip (80%), larynx (67%) and oral cavity (59%). JCV viral load was highest in larynx but variation was wide (152514 mean copies/μg DNA, SD± 304820). BKV was found only in one oral carcinoma with low viral load. SV40 was detected in 60% lip and 20.7% oral carcinomas with low copy numbers (6.6- 23.7 copies/μg DNA). Altogether, 86% of JCV-positive samples were co-infected with HPV (p=0.001), with no impact on DSS. Agreement between qPCR and Multiplex methods was substantial (Cohen's kappa= 0.659). Multiplex method detected additional HPyV in five samples. JCV was found in 9/24 HNSCC cell lines, all deriving from oral cavity. Our data provide evidence that JCV might have a role in HNSCC as independent virus or co-factor of HPV.


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Last updated on 2022-07-04 at 16:51