G5 Doctoral dissertation (article)
Interplay between adenosine receptors and adenosine deaminases

List of Authors: Liu. Chengqian
Publisher: University of Turku
Place: Turku
Publication year: 2018
ISBN: 978-951-29-7236-4
eISBN: 978-951-29-7237-1


Adenosine deaminases (ADAs) are key enzymes involved in purine metabolism and immune system. Human have two distinct adenosine deaminase isoforms, ADA1 and ADA2. ADA1 deficiency was reported as the first discovered molecular cause of human severe combined immunodeficiency (SCID) in 1972. In 2014, it was reported ADA2 mutations could cause early-onset stroke and systemic vasculopathy. However, the underlying mechanisms causing the symptoms present in the deficiency of ADA2 (DADA2) patients remain undefined. 

This thesis is focused on the function of human ADAs in the immune system and the application of human ADA1 in sandwich enzyme-linked immunosorbent assay (ELISA). The data showed that ADA1 and ADA2 bind to different subsets of immune cells and modulate their response to extracellular adenosine by interplaying with adenosine receptors. Deficiency in human ADA2 may alter cellular responses to extracellular adenosine. In addition, both ADA1 and horseradish peroxidase (HRP) used in sandwich ELISA gave identical results, suggesting that ADA1 could be used as an alternative detection enzyme to measure antigen concentrations in biological fluids. 

In summary, this thesis provides new data on the mechanisms causing those vascular inflammatory phenotypes of ADA2 mutations and suggests that ADA1 can be used as a valuable enzyme for amplification in sandwich ELISA.

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Last updated on 2019-20-07 at 06:32