Refereed journal article or data article (A1)

Hot electron-induced electrochemiluminescence of calcein and calcein-Tb(III) complex at disposable oxide-covered aluminum and polyvinyl butyral-carbon black/metal composite electrodes in aqueous solutions




List of AuthorsKalle Salminen, Päivi Grönroos, Harri Härmä, Sakari Kulmala

PublisherPERGAMON-ELSEVIER SCIENCE LTD

Publication year2018

JournalElectrochimica Acta

Journal name in sourceELECTROCHIMICA ACTA

Journal acronymELECTROCHIM ACTA

Volume number266

Start page212

End page219

Number of pages8

ISSN0013-4686

DOIhttp://dx.doi.org/10.1016/j.electacta.2018.02.016


Abstract
Hot electron-induced electrochemiluminescence (HECL) of calcein and calcein-Tb(III) complex was generated at oxide-covered aluminum electrode during cathodic pulse polarization. The excitation of calcein as a molecule or as a ligand is based on subsequent one-electron oxidation and reduction steps by oxidizing radicals and solvated electrons. During HECL excitation of calcein-Tb(III) solution a reaction product of the calcein is formed that also enables the photoexcitation of Tb(III) via the formed ligand derivative by ligand-sensitized mechanism. The determination of low concentrations of calcein with aluminum electrodes was complicated by a relatively strong background electroluminescence originating from the Al2O3-layer. Polyvinyl butyral-carbon black composite electrodes coated on brass were fabricated to solve this problem and a fifty-fold lower background emission was obtained for these novel composite electrodes in comparison to that of oxide-covered 99.9% pure aluminum electrodes. The obtained detection limits were 3.2.10(-10) M for calcein and 6.4.10(-9) M for calcein-Tb(III) at the present composite electrodes. These species could potentially be utilized as electrochemiluminescent labels in bioaffinity assays. (C) 2018 Elsevier Ltd. All rights reserved.


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