B2 Book chapter
Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture

List of Authors: Natalija Eigeliene, Risto Erkkola, Pirkko Härkönen
Publication year: 2016
Book title *: Estrogen Receptors
Title of series: Methods in Molecular Biology
Volume number: 1366
ISBN: 978-1-4939-3127-9
ISSN: 1064-3745


Explant tissue culture provides a model for studying the direct effects of steroid hormones, their analogs, and novel hormonally active compounds on normal freshly isolated human breast tissues (HBTs). For this purpose, pre- and postmenopausal HBTs can be maintained in this culture system. The results demonstrate that the morphological integrity of HBT explants can be maintained in tissue culture up to 2 weeks and expression of differentiation markers, steroid hormone receptors, proliferation and apoptosis ratios can be evaluated as a response to hormonal stimulation. This chapter describes an ex vivo culture model that we have applied to study the effects of various hormonally active substances, including 17β-estradiol and selective estrogen receptor modulators (SERMs), on normal human breast tissues.

Last updated on 2019-21-08 at 22:18