A1 Journal article – refereed
Gestational hyperglycemia reprograms cardiac gene expression in rat offspring

List of Authors: Lehtoranta L, Koskinen A, Vuolteenaho O, Laine J, Kyto V, Soukka H, Ekholm E, Rasanen J
Publication year: 2017
Journal: Pediatric Research
Journal name in source: PEDIATRIC RESEARCH
Journal acronym: PEDIATR RES
Volume number: 82
Issue number: 2
Number of pages: 6
ISSN: 0031-3998
eISSN: 1530-0447

BACKGROUND: Rat fetuses with maternal pregestational hyperglycemia develop cardiac dysfunction, and their cardiac gene expression differs from that of healthy control fetuses near term. We hypothesized that cardiac gene expression and morphologic abnormalities of rat fetuses with maternal pregestational hyperglycemia become normal after birth.METHODS: Nine rats were preconceptually injected with streptozotocin to induce maternal hyperglycemia and nine rats served as controls. The hyperglycemia group comprised 82 mice and the control group 74 offspring fed by euglycemic dams. Hearts of the offspring were collected on postnatal days 0, 7, and 14, and processed for histologic and gene expression analyses.RESULTS: On day 0, heart weight was increased, and expression of cardiac genes involved in contractility, growth, and metabolism was decreased in the hyperglycemia group. On day 7, although cardiomyocyte apoptosis was enhanced, most of the changes in gene expression had normalized in the hyperglycemia group. By day 14, the expression of genes important for myocardial growth, function, and metabolism was again abnormal in the hyperglycemia group.CONCLUSION: Most cardiac gene expression abnormalities become transiently normal during the first week of life of offspring to hyperglycemic rats. However, by day 14, cardiac expressions of genes involved in growth, function, and metabolism are again abnormal in relation to control offspring.

Last updated on 2019-30-07 at 17:28