A1 Journal article – refereed
T cell fate and clonality inference from single-cell transcriptomes

List of Authors: Stubbington MJT, Lonnberg T, Proserpio V, Clare S, Speak A, Dougan G, Teichmann SA
Publication year: 2016
Journal: Nature Methods
Journal name in source: NATURE METHODS
Journal acronym: NAT METHODS
Volume number: 13
ISSN: 1548-7091

We developed TraCeR, a computational method to reconstruct full-length, paired T cell receptor (TCR) sequences from T lymphocyte single-cell RNA sequence data. TraCeR links T cell specificity with functional response by revealing clonal relationships between cells alongside their transcriptional profiles. We found that T cell clonotypes in a mouse Salmonella infection model span early activated CD4(+) T cells as well as mature effector and memory cells.

Last updated on 2019-20-07 at 04:28