A1 Journal article – refereed
Communication Between the Calcium and cAMP Pathways Regulate the Expression of the TSH Receptor: TRPC2 in the Center of Action

List of Authors: Lof C, Sukumaran P, Viitanen T, Vainio M, Kemppainen K, Pulli I, Nasman J, Kukkonen JP, Tornquist K
Publication year: 2012
Journal: Molecular Endocrinology -Baltimore-
Journal name in source: MOLECULAR ENDOCRINOLOGY
Journal acronym: MOL ENDOCRINOL
Number in series: 12
Volume number: 26
Issue number: 12
Number of pages: 12
ISSN: 0888-8809

Transient receptor potential (TRP) cation channels are widely expressed and function in many physiologically important processes. Perturbations in the expression or mutations of the channels have implications for diseases. Many thyroid disorders, as excessive growth or disturbed thyroid hormone production, can be a result of dysregulated TSH signaling. In the present study, we found that of TRP canonicals (TRPCs), only TRPC2 was expressed in Fischer rat thyroid low-serum 5% cells (FRTL-5 cells). To investigate the physiological importance of the channel, we developed stable TRPC2 knockdown cells using short hairpin RNA (shTRPC2 cells). In these cells, the ATP-evoked entry of calcium was significantly decreased. This led to increased cAMP production, because inhibitory signals from calcium to adenylate cyclase 5/6 were decreased. Enhanced cAMP signaling projected to Ras-related protein 1-MAPK kinase 1 (MAPK/ERK kinase 1) pathway leading to phosphorylation of ERK1/2. The activated ERK1/2 pathway increased the expression of the TSH receptor. In contrast, secretion of thyroglobulin was decreased in shTRPC2 cells, due to improper folding and glycosylation of the protein. We show here a novel role for TRPC2 in regulating thyroid cell function. (Molecular Endocrinology 26: 2046-2057, 2012)

Internal Authors/Editors

Last updated on 2019-20-07 at 08:44