G5 Doctoral dissertation (article)
PV-1: Leukocyte trafficking molecule and vascular marker

List of Authors: Keuschnigg Johannes
Publisher: University of Turku
Place: Turku
Publication year: 2013
ISBN: 978-951-29-5405-6


The distinction between lymphatic vessels and blood vessels is a crucial factor in

many studies in immunology, vascular biology and cancer biology. They both share

several characteristics and perform related, though different functions. They are

equally important for the performance of the human immune system with the

continuous recirculation of leukocytes from the tissues via lymphatics to the blood

vessels and back into the tissue presenting the link between both systems.

This study was undertaken to elucidate the differences in the gene expression between

primary blood- and lymphatic endothelial cells as well as the two immortalized cell

lines HMEC-1 (human microvascular endothelial cell line 1) and TIME (telomerase

immortalized microvascular endothelial cell line). Furthermore, we wanted to

investigate the mystery surrounding the identity of the antigen recognized by the

prototype blood vascular marker PAL-E. In the last step we wanted to study whether

the PAL-E antigen would be involved in the process of leukocyte migration from the

bloodstream into the surrounding tissue.

Our results clearly show that the gene expression in primary blood endothelial cells

(BEC), lymphatic endothelial cells (LEC) and the cell lines HMEC-1 and TIME is

plastic. Comparison of a large set of BEC- and LEC datasets allowed us to assemble a

catalog of new, stable BEC- or LEC specific markers, which we verified in

independent experiments. Additionally, several lines of evidence demonstrated that

PAL-E recognizes plasmalemma vesicle associated protein 1 (PV-1), which can form

complexes with vimentin and neuropilin-1. Finally, numerous in vitro and in vivo

experiments identify the first function of the protein PV-1 during leukocyte

trafficking, where it acts as regulatory molecule.

Internal Authors/Editors

Last updated on 2019-21-08 at 20:07