Refereed journal article or data article (A1)

The time course of L-gulono-gamma-lactone oxidase expression during the larval ontogeny of fish (Acipenser persicus)




List of AuthorsAkbarzadeh A, Haghbeen K, Farahmand H, Nematollahi MA, Nikinmaa M

PublisherTAYLOR & FRANCIS LTD

PlaceLondon

Publication year2013

JournalMarine and Freshwater Behaviour and Physiology

Journal name in sourceMARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY

Journal acronymMAR FRESHW BEHAV PHY

Number in series5

Volume number45

Issue number5

Start page349

End page355

Number of pages7

ISSN1023-6244

DOIhttp://dx.doi.org/10.1080/10236244.2013.768002


Abstract
In our previous study, for the first time in any fish species, the mRNA expression of L-Gulono--lactone oxidase (GULO) was investigated during early development of Persian sturgeon, Acipenser persicus, and it was demonstrated that GULO mRNA was expressed throughout the development of Persian sturgeon. Accordingly, it was suggested that the initial production of GULO and probably ascorbate might occur before hatching during the embryonic stage. To evaluate the correspondence between transcript and protein level, GULO activity of Persian sturgeon during ontogenetic development of embryos and larvae was investigated. The GULO activity was detected in all time-points of the development of Persian sturgeon from embryos to the late larval stage indicating the start of ascorbate synthesis before hatching. However, there was a marked difference in the mRNA/protein activity ratio at different times. As the GULO mRNA and protein production peaks were observed during a time course from embryonic to early larval development before the start of the exogenous feeding, it can be concluded that the vitamin C requirement must initially be fulfilled by endogenous formation.


Last updated on 2021-24-06 at 11:56