A1 Journal article – refereed
The cleavage of phosphodiester bonds within small RNA bulges in the presence and absence of metal ion catalysts




List of Authors: Kaukinen U, Bielecki L, Mikkola S, Adamiak RW, Lonnberg H
Publisher: ROYAL SOC CHEMISTRY
Publication year: 2001
Journal: Journal of the Chemical Society, Perkin Transactions 2
Journal name in source: JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
Journal acronym: J CHEM SOC PERK T 2
Issue number: 7
Number of pages: 8
ISSN: 1472-779X

Abstract
This work studies the effect of RNA structure on the reactivity of RNA phosphodiester bonds. The aim of the study is to evaluate those structural parameters that affect reactivity to support the development of sequence-specific RNA cleaving agents. The cleavage of phosphodiester bonds within one- to five-nucleotide bulges has been studied in buffer solutions (pH 8.4), and in the presence of Zn2+ aqua ion (pH 6.4) or its 1,5,9-triazacyclododecane complex (pH 7.4). Molecular modelling has been applied to study the structure of the substrates. The basis of the reactivity and the catalytic potential of metal ion-based cleaving agents are discussed.


Research Areas


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