A1 Journal article – refereed
2 '-O-[(4-CF3-triazol-1-yl)methyl] Uridine - A Sensitive F-19 NMR Sensor for the Detection of RNA Secondary Structures




List of Authors: Granqvist L, Virta P
Publisher: AMER CHEMICAL SOC
Publication year: 2015
Journal: Journal of Organic Chemistry
Journal name in source: JOURNAL OF ORGANIC CHEMISTRY
Journal acronym: J ORG CHEM
Volume number: 80
Issue number: 16
ISSN: 0022-3263
eISSN: 1520-6904

Abstract


A sensitive uridine-derived sensor (viz., 2'-O-[(4-CF3-triazol-1-yOmethyl]uridine, 1) for F-19 NMR spectroscopic monitoring of RNA secondary structures is described. The applicability of 1 is demonstrated by monitoring the thermal denaturation of the following double and triple helical RNA models: (1) a miR 215 hairpin, (2) a poly U-A*U triple helix RNA (bearing two C-G*CH+ interrupts), and (3) a polyadenylated nuclear-nuclear retention element complex. In these RNA models, the F-19 NMR shift of the 2'-O-(CF3-triazolylmethyl) group shows high sensitivity to secondary structural arrangements. Moreover, 1 favors the desired N-conformation, and its effect on both RNA duplex and triplex stabilities is marginal.



Last updated on 2019-29-01 at 12:40