A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
16S rDNA PCR-denaturing gradient gel electrophoresis in detennining proportions of coexisting Actinobacillus actinomycetemcomitans strains
Tekijät: Ihalin R, Asikainen S
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2006
Journal: Journal of Microbiological Methods
Tietokannassa oleva lehden nimi: JOURNAL OF MICROBIOLOGICAL METHODS
Lehden akronyymi: J MICROBIOL METH
Vuosikerta: 65
Numero: 3
Aloitussivu: 417
Lopetussivu: 424
Sivujen määrä: 8
ISSN: 0167-7012
DOI: https://doi.org/10.1016/j.mimet.2005.08.016
Verkko-osoite: https://www.sciencedirect.com/science/article/pii/S0167701205002769?via=ihub
Tiivistelmä
Certain serotypes of Actinobacillus actinomycetemcomitans seem to prefer coexistence in vivo. The 16S rDNA PCR-denaturing gradient gel electrophoresis (DGGE) was tested for its capability to distinguish coexisting A. actinomycetemcomitans strains of different serotypes or genetic lineages and to determine their proportions in vitro. The migration pattern of the PCR amplicon from serotype c differed from those of the other serotypes. Contrary to the strains of serotypes c, d, and e, strains of serotypes a, b, and f consistently demonstrated intra-serotype migration patterns similar to each other. Since the migration patterns differed between serotype c and b strains a strain of each was used to determine their proportional representation in a strain mixture. The strains were distinguishable from each other above the 5% PCR-DGGE detection level (12.5 ng DNA/1.5 x 10(6) cells). DGGE provides a promising tool for in vitro studies on the coexistence of different genetic lineages of A. actinomycetemcomitans. (c) 2005 Elsevier B.V. All rights reserved.
Certain serotypes of Actinobacillus actinomycetemcomitans seem to prefer coexistence in vivo. The 16S rDNA PCR-denaturing gradient gel electrophoresis (DGGE) was tested for its capability to distinguish coexisting A. actinomycetemcomitans strains of different serotypes or genetic lineages and to determine their proportions in vitro. The migration pattern of the PCR amplicon from serotype c differed from those of the other serotypes. Contrary to the strains of serotypes c, d, and e, strains of serotypes a, b, and f consistently demonstrated intra-serotype migration patterns similar to each other. Since the migration patterns differed between serotype c and b strains a strain of each was used to determine their proportional representation in a strain mixture. The strains were distinguishable from each other above the 5% PCR-DGGE detection level (12.5 ng DNA/1.5 x 10(6) cells). DGGE provides a promising tool for in vitro studies on the coexistence of different genetic lineages of A. actinomycetemcomitans. (c) 2005 Elsevier B.V. All rights reserved.
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