A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
RNA polymerase gate loop guides the nontemplate DNA strand in transcription complexes




Julkaisun tekijät: NandyMazumdar M, Nedialkov Y, Svetlov D, Sevostyanova A, Belogurov GA, Artsimovitch I
Kustantaja: NATL ACAD SCIENCES
Julkaisuvuosi: 2016
Tietokannassa oleva lehden nimi: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Lehden akronyymi: P NATL ACAD SCI USA
Volyymi: 113
Julkaisunumero: 52
Sivujen määrä: 6
ISSN: 0027-8424
eISSN: 1091-6490

Tiivistelmä
Upon RNA polymerase (RNAP) binding to a promoter, the s factor initiates DNA strand separation and captures the melted nontemplate DNA, whereas the core enzyme establishes interactions with the duplex DNA in front of the active site that stabilize initiation complexes and persist throughout elongation. Among many core RNAP elements that participate in these interactions, the beta' clamp domain plays the most prominent role. In this work, we investigate the role of the beta gate loop, a conserved and essential structural element that lies across the DNA channel from the clamp, in transcription regulation. The gate loop was proposed to control DNA loading during initiation and to interact with NusG-like proteins to lock RNAP in a closed, processive state during elongation. We show that the removal of the gate loop has large effects on promoter complexes, trapping an unstable intermediate in which the RNAP contacts with the nontemplate strand discriminator region and the downstream duplex DNA are not yet fully established. We find that although RNAP lacking the gate loop displays moderate defects in pausing, transcript cleavage, and termination, it is fully responsive to the transcription elongation factor NusG. Together with the structural data, our results support a model in which the gate loop, acting in concert with initiation or elongation factors, guides the nontemplate DNA in transcription complexes, thereby modulating their regulatory properties.


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Last updated on 2019-29-01 at 15:45