Refereed journal article or data article (A1)

Molecular identification of PAL-E, a widely used endothelial-cell marker




List of AuthorsNiemela H, Elima K, Henttinen T, Irjala H, Salmi M, Jalkanen S

PublisherAMER SOC HEMATOLOGY

Publication year2005

JournalBlood

Journal name in sourceBLOOD

Journal acronymBLOOD

Volume number106

Issue number10

Start page3405

End page3409

Number of pages5

ISSN0006-4971

DOIhttp://dx.doi.org/10.1182/blood-2005-01-0254


Abstract
The pathologische anatomie Leiden-endothelium (PAL-E) antibody has been used for almost 20 years as a specific marker for vascular endothelial cells. Due to the fact that this antibody works only in very limited applications, the molecular identity of PAL-E has remained unknown. In this work, we demonstrate by double stainings, cross-immunoprecipitations, and transfectants that the PAL-E antigen is identical with a protein designated PV-1 (plasmalemmal vesicle 1) or FELS (fenestrated endothelial-linked structure protein) and is not vimentin, as reported earlier. As the expression of this molecule is by no means restricted to fenestrated endothelium, we suggest the use of the name PLVAP for this protein. Molecular identification of PLVAP should help in the production of new tools for the identification of vascular as opposed to lymphatic endothelium and to elucidate the function of this protein.


Last updated on 2021-24-06 at 12:02