A1 Journal article – refereed
Vimentin function in lymphocyte adhesion and transcellular migration




List of Authors: Nieminen M, Henttinen T, Merinen M, Marttila-Ichihara F, Eriksson JE, Jalkanen S
Publisher: NATURE PUBLISHING GROUP
Publication year: 2006
Journal: Nature Cell Biology
Journal name in source: NATURE CELL BIOLOGY
Journal acronym: NAT CELL BIOL
Volume number: 8
Issue number: 2
ISSN: 1465-7392

Abstract
Although the adhesive interactions of leukocytes with endothelial cells are well understood, little is known about the detailed mechanisms underlying the actual migration of leukocytes across the endothelium ( diapedesis). Leukocytes have been shown to use both paracellular and transcellular routes for transendothelial migration. Here we show that peripheral blood mononuclear cells (PBMCs; T- and B-lymphocytes) preferentially use the transcellular route. The intermediate filaments of both endothelial cells and lymphocytes formed a highly dynamic anchoring structure at the site of contact between these two cell types. The initiation of this process was markedly reduced in vimentin-deficient (vim(-/-)) PBMCs and endothelial cells. When compared with wild-type PBMCs, vim(-/-) PBMCs showed a markedly reduced capacity to home to mesenteric lymph nodes and spleen. Furthermore, endothelial integrity was compromised in vim(-/-) mice, demonstrating that intermediate filaments also regulate the barrier that governs leukocyte extravasation. Absence of vimentin resulted in highly aberrant expression and distribution of surface molecules critical for homing (ICAM-1 and VCAM-1 on endothelial cells and integrin-beta 1 on PBMCs). These data show that intermediate filaments are active in lymphocyte adhesion and transmigration.

Last updated on 2019-21-08 at 23:31