A1 Journal article – refereed
Alveolar bone loss in relation to toll-like receptor 4 and 9 genotypes and Porphyromonas gingivalis carriage.

List of Authors: Ulvi Kahraman Gursoy, Qiushui He, Pirkko Pussinen, Sisko Huumonen, Eija Könönen
Publication year: 2016
Journal: European Journal of Clinical Microbiology and Infectious Diseases
Volume number: 35
Issue number: 11
Number of pages: 6


Toll-like receptors (TLRs) are highly developed sensors to detect
microbe-associated molecular patterns. Functional polymorphisms of the
genes TLR4 and TLR9 were found to be associated with alveolar bone loss
in a Porphyromonas gingivalis-induced periodontitis model in mice. Our
aim was to examine whether such an association can be detected in a
group of Finnish adults. Polymorphisms of TLR4 Asp299Gly (rs4986790) and
TLR9 rs187084 (1486 T/C) were genotyped by pyrosequencing and PCR from
the saliva samples of 223 adults (age range 40-60 years). Alveolar bone
loss, measured from panoramic radiographs, were compared between TLR
genotype groups according to subjects' salivary carriage of P.
gingivalis, measured using a single copy gene-based real-time PCR. The
frequencies of TLR4 wild type and heterozygote variants were 87.4 % and
12.6 %, respectively, while those of TLR9 wild type, heterozygote, and
homozygote variants were 25.6 %, 39.1 %, and 35.3 %, respectively. In
the TLR4 heterozygote group, P. gingivalis-positive subjects had more
alveolar bone loss than P. gingivalis-negative subjects (p = 0.027),
while no difference was observed in the wild type group. P.
gingivalis-negative individuals with TLR9 heterozygotes exhibited
significantly less alveolar bone loss compared to those with TLR9 wild
type (p = 0.007). Polymorphisms of TLR4 in P. gingivalis carriers seem
to expose to alveolar bone loss. Polymorphisms of TLR9 can be protective
against alveolar bone loss in the absence of P. gingivalis.

Last updated on 2019-21-08 at 21:25