A1 Journal article – refereed
Assessment of stress tolerance acquisition in the heat-tolerant derivative strains of Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus rhamnosus GG

List of Authors: Aakko J, Sanchez B, Gueimonde M, Salminen S
Publisher: The Society for Applied Microbiology
Publication year: 2014
Journal: Journal of Applied Microbiology
Journal name in source: Journal of applied microbiology
Journal acronym: J.Appl.Microbiol.
Volume number: 117
Issue number: 1
Number of pages: 10
ISSN: 1364-5072


AIMS: The purpose of this study was to investigate the heat-shock response at molecular level in Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. lactis BB-12 and their heat-tolerant derivatives and to characterize the changes that make the derivatives more robust in terms of heat stress. METHODS AND RESULTS: The study strains were exposed for 2 h to a heat-shock treatment, Bif. animalis subsp. lactis BB-12 and its derivative at 50 degrees C and the Lact. rhamnosus GG and its derivative at 60 degrees C. Protein synthesis before and after heat shock was examined using proteomics and RT-qPCR. The analysis revealed that the regulation of seven proteins in both strain pairs was modified as a response to heat or between the original and the derivative strain. The comparison of wild-type strains and the heat-tolerant derivatives suggests that the acquisition of heat tolerance in the Bif. animalis subsp. lactis BB-12 derivative is due to a slightly increased constitutive level of chaperones, while in Lact. rhamnosus GG derivative, the main reason seems to be a higher ability to induce the production of chaperones. CONCLUSIONS: This study revealed possible markers of heat tolerance in B. lactis and Lact. rhamnosus strains. SIGNIFICANCE AND IMPACT OF STUDY: This study increases our knowledge on how Lactobacillus and Bifidobacterium strains may acquire heat tolerance. These findings may be useful for improving the heat tolerance of existing probiotic strains as well as screening new heat-tolerant strains.

Last updated on 2019-20-07 at 06:04