Robert Moulder
PhD, CChem, MRSC

Senior Researcher, Centre for Biotechnology (Turku Centre for Biotechnology)

robmou@utu.fi
+358 29 450 3802
Tykistökatu 6
Turku
Office: 7115




Areas of expertise
Chromatography, Mass spectrometry, proteomics, protein biomarkers, analytical chemistry, type 1 diabetes, T-helper cells, bioanalysis, supercritical fluids

Biography
Following PhD studies at the University of Leeds on analytical applications of supercritical fluids, I combined these methodologies with mass spectrometry in biomarker research as a postdoctoral researcher at the Institute of Chemistry at Uppsala University, Sweden. From the chemistry department I moved to the Positron Emission Tomography facility at Uppsala University hospital where I worked as senior analytical chemist in a variety of projects including tracer quality control, development and metabolite analysis.
I moved to Finland to work with bioanalytics at Orion-Pharma, then back into academia at the University of Turku in the proteomics core facility at Turku Centre for Biotechnology. From the core facility I moved to the Molecular Immunology group of Professor Lahesmaa where I have used proteomics in the study of T helper cell subsets, in addition to biomarker studies of type 1 diabetes, sarcoidosis, atherosclerosis and ovarian cancer.


Research
With recent improvements in chromatographic and mass spectrometry technology it has been increasingly possible to provide detailed proteomic information of complex samples in a time efficient manner. Such characterizations of biological systems can provide important insight into cell biology and disease pathologies. Following this theme, my research pursuits are divided into two related categories, proteomic biomarker identification and proteomics characterization of cells pertinent to the immune system.

Using samples collected in the Finnish Diabetes Prediction and Prevention (DIPP) study I have used mass spectrometry based quantitative proteomics to identify early changes and risk related differences in the temporal proteomes of children that develop type 1 diabetes in comparison with age, gender and risk matched controls. Our publication of this research presented the first detailed temporal proteome of children at risk from T1D. Following on from this work further validations in independent cohorts are in progress, in addition to other T1D research and related studies. Similarly, using longitudinal samples we have studied the differences in the serum protein profiles of subjects with asymptomatic cardiovascular atherosclerosis.

The key genetic component for T1D is found amongst variants of the human leucocyte antigen (HLA) gene, the functions of which include activatation of CD4+ T cells. As inappropriate CD4+ T helper cell activation and polarization have been linked with several inflammatory and autoimmune diseases, an understanding of the molecular mechanisms of these processes is important in identifying the pathologies of related disease states. We have used quantitative proteomics to study the early changes in the differentiation process, including published proteomics works on Th2 and Th1 cells, including complementary genomics. Ongoing work concern the use of targeted and non-targeted proteomics to analyse these and other Th cell subsets, detailing protein interactions and temporal profiles.


Publications
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Quantitative Proteomics Reveals the Dynamic Protein Landscape during Initiation of Human Th17 Cell Polarization (2019) Tripathi SK, Välikangas T, Shetty A, Khan MM, Moulder R, Bhosale SD, Komsi E, Salo V, De Albuquerque RS, Rasool O, Galande S, Elo LL, Lahesmaa R
(
A1 Journal article – refereed)

Characterization and non-parametric modeling of the developing serum proteome during infancy and early childhood (2018)
Scientific Reports
Niina Lietzén, Lu Cheng, Robert Moulder, Heli Siljander, Essi Laajala, Taina Härkönen, Aleksandr Peet, Aki Vehtari, Vallo Tillmann, Mikael Knip, Harri Lähdesmäki, Riitta Lahesmaa
(
A1 Journal article – refereed)

Proteomics of Diabetes, Obesity, and Related Disorders (2018)
PROTEOMICS - Clinical Applications
Robert Moulder, Domitille Schvartz, David R. Goodlett, Loïc Dayon
(
B1 Journal article)

Quantitative proteomic characterization and comparison of T helper 17 and induced regulatory T cells (2018)
PLoS Biology
Imran Mohammad, Kari Nousiainen, Santosh D. Bhosale, Inna Starskaia, Robert Moulder, Anne Rokka, Fang Cheng, Ponnuswamy Mohanasundaram, John E. Eriksson, David R. Goodlett, Harri Lähdesmäki, Zhi Chen
(
A1 Journal article – refereed)

Serum Proteomic Profiling to Identify Biomarkers of Premature Carotid Atherosclerosis (2018)
Scientific Reports
Santosh D. Bhosale, Robert Moulder, Mikko S. Venäläinen, Juhani S. Koskinen, Niina Pitkänen, Markus T. Juonala, Mika A. P. Kähönen, Terho J. Lehtimäki, Jorma S. A. Viikari, Laura L. Elo, David R. Goodlett, Riitta Lahesmaa, Olli T. Raitakari
(
A1 Journal article – refereed)

Analysis of the plasma proteome using iTRAQ and TMT-based Isobaric labeling (2017)
Mass Spectrometry Reviews
Robert Moulder, Santosh D. Bhosale, David R. Goodlett, Riitta Lahesmaa
(
A2 Review article in a scientific journal)

DNA methylation and Transcriptome Changes Associated with Cisplatin Resistance in Ovarian Cancer (2017)
Scientific Reports
Riikka J. Lund, Kaisa Huhtinen, Jussi Salmi, Juha Rantala, Elizabeth V. Nguyen, Robert Moulder, David R. Goodlett, Riitta Lahesmaa, Olli Carpén
(
A1 Journal article – refereed)

Mass Spectrometry-based Serum Proteomics for Biomarker discovery and validation (2017) Serum/plasma proteomics : Methods and Protocols Santosh D. Bhosale, Robert Moulder, Petri Kouvonen, Riitta Lahesmaa, David R. Goodlett
(
A3 Book chapter)

Pulmonary sarcoidosis is associated with exosomal vitamin D-binding protein and inflammatory molecules (2017)
Journal of Allergy and Clinical Immunology
Maria-Jose Martinez-Bravo, Casper J.E. Wahlund, Khaleda Rahman Qazi, Robert Moulder, Ana Lukic, Olof Rådmark, Riitta Lahesmaa, Johan Grunewald, Anders Eklund, Susanne Gabrielsson
(
A1 Journal article – refereed)

The progress and potential of proteomic biomarkers for type 1 diabetes in children (2017)
Expert Review of Proteomics
Robert Moulder, Santosh D. Bhosale, Riitta Lahesmaa, David R. Goodlett
(
A2 Review article in a scientific journal)

Early signs of disease in type 1 diabetes (2016)
Pediatric Diabetes
Robert Moulder, Riitta Lahesmaa
(
A2 Review article in a scientific journal)

Label-Free Quantitation for Clinical Proteomics (2016) Quantitative Proteomics by Mass Spectrometry Robert Moulder, Young Ah Goo, David R. Goodlett
(
A3 Book chapter)

New Insights and Biomarkers for Type 1 Diabetes: Review for Scandinavian Journal of Immunology (2015)
Scandinavian Journal of Immunology
Heinonen MT, Moulder R, Lahesmaa R
(
A2 Review article in a scientific journal)

Quantitative Analysis of the Erythrocyte Membrane Proteins in Polycythemia Vera Patients on Treated with Hydroxycarbamide (2015) Kottahachchi D, Gooneratne L, Jayasekera A, Muth-Pawlak D, Moulder R, Imanishi S, Ariyaratne A, Rokka A, Corthals G,
(
A1 Journal article – refereed)

Serum Proteomes Distinguish Children Developing Type 1 Diabetes in a Cohort With HLA-Conferred Susceptibility (2015)
Diabetes
Moulder R, Bhosale SD, Erkkila T, Laajala E, Salmi J, Nguyen EV, Kallionpaa H, Mykkanen J, Vaha-Makila M, Hyoty H, Veijola R, Ilonen J, Simell T, Toppari J, Knip M, Goodlett DR, Lahdesmaki H, Simell O, Lahesmaa R
(
A1 Journal article – refereed)

The human diabetes proteome project (HDPP): The 2014 update (2015) Schvartz D, Bergsten P, Baek K.-H, Barba De La Rosa A, Cantley J, Dayon L, Finamore F, Fontana P, Gaudet P, Goo Y-A, Moulder R, Goodlett D, Johnson J.D, Konvalinka A, Mulder H, Priego-Capote F, Sechi S, Snyder M, Tiss A, Wiederkehr A, Xenarios I, Kussmann M, Sanchez J-C
(
A1 Journal article – refereed)

The L1TD1 Protein Interactome Reveals the Importance of Post-transcriptional Regulation in Human Pluripotency (2015)
Stem Cell Reports
Emani MR, Närvä E, Stubb A, Chakroborty D, Viitala M, Rokka A, Rahkonen N, Moulder R, Denessiouk K, Trokovic R, Lund R, Elo LL, Lahesmaa R.
(
A1 Journal article – refereed)

No association between vitamin D and β-cell autoimmunity in Finnish and Estonian children (2014)
Diabetes/Metabolism Research and Reviews
L. Reinert-Hartwall, J. Honkanen, T. Härkönen, J. Ilonen, O. Simell, A. Peet, V. Tillmann, C. Lamberg-Allardt, S. M. Virtanen, M. Knip, O. Vaarala, The DIABIMMUNE study Group
(
A1 Journal article – refereed)

Standard of hygiene and immune adaptation in newborn infants. (2014)
Clinical Immunology
Kallionpää Henna, Laajala Essi, Öling Viveka, Härkönen Taina, Tillmann Vallo, Dorshakova Natalya V, Ilonen Jorma, Lähdesmäki Harri, Knip Mikael, Lahesmaa Riitta; DIABIMMUNE Study Group
(
A1 Journal article – refereed)

Statistical Analysis of Protein microarray Data: a case study in type 1 diabetes research (2014)
Journal of Proteomics and Bioinformatics
Le Thi Thanh An, Anna Pursiheimo, Robert Moulder, and Laura L. Elo
(
A2 Review article in a scientific journal)


Last updated on 2019-27-05 at 02:19