Georgi Belogurov
PhD, Docent, Adjunct Professor

erikoistutkija sr., Biokemia (Biokemian laitos)
dosentti, Biokemia (Biokemian laitos)

gebelo@utu.fi
+358 29 450 4775
+358 50 475 7149

Tykistökatu 6
Turku
Arcanum
Työhuone: K112





Asiantuntijuusalueet
Transcription, enzyme kinetics, protein chemistry, phylogenomics.

Biografia


M.S.- Moscow State University, School of Chemistry, Russia, 1997
Ph.D. - University of Turku, Dept. of Biochemistry and Food Chemistry, Finland, 2005
Postdoc - Ohio State Univesity, Dept. of Microbiology, USA, 2006-2009
Principal investigator - University of Turku, Dept. of Biochemistry, Finland, 2009-



Tutkimus

RNA polymerase (RNAP) is the enzyme that carries out the first step in gene expression, synthesis of RNA. All cellular RNAPs are multisubunit enzymes sharing homologous catalytic cores totaling ~2,500 amino acids. During RNA synthesis RNAP functions as a molecular motor and a helicase; its movement along the DNA is ultimately powered by the free energy liberated as nucleoside triphosphates are condensed into the nascent RNA and pyrophosphate is released. By importance and conservation among the life kingdoms RNAP rivals the ribosome, yet is considerably less complex and is amenable to kinetics and mechanistic studies in well-defined in vitro systems.

We study RNAP catalytic mechanism with emphasis on translocation, a step specific to processive polymerases and motor enzymes. Utilizing a relatively simple bacterial RNAP system we address mechanical, kinetic and energetic aspects of RNAP movement along the DNA. Another related line of our research deals with transcription elongation factors. Understanding the means by which transcription factors modulate RNAP catalytic efficiency and translocation kinetics helps us to unravel the basic mechanism of this complex molecular machine. Finally, RNAP is both an excellent phylogenetic marker for inferring species phylogenies and an attractive paradigm for studying the protein evolution. We employ molecular phylogenetic analyses to study the evolution of protein landscape in catalytically important areas of RNAP, as well as the evolution of other transcription-related proteins and the whole organisms.


Opetus

Molecular Biology (M. Sci. level)



Julkaisut
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Active site closure stabilizes the backtracked state of RNA polymerase (2018)
Nucleic Acids Research
Turtola M, Mäkinen JJ, Belogurov GA
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Locking the nontemplate DNA to control transcription (2018)
Molecular Microbiology
Nedialkov Y, Svetlov D, Belogurov GA, Artsimovitch I
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Structural basis of RNA polymerase I stalling at UV light-induced DNA damage (2018)
Proceedings of the National Academy of Sciences of the United States of America
Sanz-Murillo M, Xu J, Belogurov GA, Calvo O, Gil-Carton D, Moreno-Morcillo M, Wang D, Fernandez-Tornero C
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Uneven Braking Spins RNA Polymerase into a Pause (2018)
Molecular Cell
Artsimovitch I, Belogurov GA
(
B1 Kirjoitus tieteellisessä aikakauslehdessä)

Discovery of the Showdomycin Gene Cluster from Streptomyces showdoensis ATCC 15227 Yields Insight into the Biosynthetic Logic of C-Nucleoside Antibiotics (2017)
ACS Chemical Biology
Kaisa Palmu, Petja Rosenqvist, Keshav Thapa, Yulia Ilina, Vilja Siitonen, Bikash Baral, Janne Mäkinen, Georgi Belogurov, Pasi Virta, Jarmo Niemi, Mikko Metsä-Ketelä
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

GreA and GreB Enhance Expression of Escherichia coli RNA Polymerase Promoters in a Reconstituted Transcription–Translation System (2016)
ACS Synthetic Biology
Lea L. de Maddalena, Henrike Niederholtmeyer, Matti Turtola, Zoe N. Swank, Georgiy A. Belogurov, and Sebastian J. Maerkl
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Lineage-specific variations in the trigger loop modulate RNA proofreading by bacterial RNA polymerases (2016)
Nucleic Acids Research
Esyunina D, Turtola M, Pupov D, Bass I, Klimašauskas S, Belogurov G, Kulbachinskiy A
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble (2016)
eLife
Matti Turtola, Georgiy A Belogurov
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

RNA polymerase gate loop guides the nontemplate DNA strand in transcription complexes (2016) NandyMazumdar M, Nedialkov Y, Svetlov D, Sevostyanova A, Belogurov GA, Artsimovitch I
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Creative Math of RNA Polymerase III Termination: Sense Plus Antisense Makes More Sense (2015)
Molecular Cell
Artsimovitch I, Belogurov GA
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Monitoring translocation of multisubunit RNA polymerase along the DNA with fluorescent base analogues (2015) Bacterial Transcriptional Control Malinen Anssi M., Turtola Matti, Belogurov Georgiy A.
(
A3 Kirjan tai muun kokoomateoksen osa)

Regulation of Transcript Elongation (2015)
Annual Review of Microbiology
Belogurov GA, Artsimovitch I
(
A2 Katsausartikkeli tieteellisessä aikauslehdessä)

CBR antimicrobials alter coupling between the bridge helix and the β subunit in RNA polymerase (2014)
Nature Communications
Malinen AM, Nandymazumdar M, Turtola M, Malmi H, Grocholski T, Artsimovitch I, Belogurov GA.
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Tracing the Evolution of Angucyclinone Monooxygenases: Structural Determinants for C-12b Hydroxylation and Substrate Inhibition in PgaE (2013)
Biochemistry
Kallio P, Patrikainen P, Belogurov GA, Mantsala P, Yang KQ, Niemi J, Metsa-Ketela M
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Active site opening and closure control translocation of multisubunit RNA polymerase (2012)
Nucleic Acids Research
Malinen AM, Turtola M, Parthiban M, Vainonen L, Johnson MS, Belogurov GA
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Na+-translocating Membrane Pyrophosphatases Are Widespread in the Microbial World and Evolutionarily Precede H+-translocating Pyrophosphatases (2011)
Journal of Biological Chemistry
Luoto HH, Belogurov GA, Baykov AA, Lahti R, Malinen AM
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

The beta Subunit Gate Loop Is Required for RNA Polymerase Modification by RfaH and NusG (2011)
Molecular Cell
Anastasia Sevostyanova, Georgiy A Belogurov, Rachel A Mooney, Robert Landick, Irina Artsimovitch
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Functional regions of the N-terminal domain of the antiterminator RfaH (2010)
Molecular Microbiology
Belogurov GA, Sevostyanova A, Svetlov V, Artsimovitch I
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Functional specialization of transcription elongation factors (2009)
EMBO Journal
Belogurov GA, Mooney RA, Svetlov V, Landick R, Artsimovitch I
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)

Transcription inactivation through local refolding of the RNA polymerase structure (2009)
Nature
Belogurov GA, Vassylyeva MN, Sevostyanova A, Appleman JR, Xiang AX, Lira R, Webber SE, Klyuyev S, Nudler E, Artsimovitch I, Vassylyev DG
(
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä)


Last updated on 2019-26-05 at 02:17